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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
28/11/2019 |
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Data da última atualização: |
28/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARCIANO, C. M. M.; IBELLI, A. M. G.; PEIXOTO, J. de O.; SAVOLDI, I. R.; CARMO, C. B. do; FERNANDES, L. T.; LEDUR, M. C. |
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Afiliação: |
CAROLINE M. MARINHO MARCIANO, UDESC/Chapecó; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; JANE DE OLIVEIRA PEIXOTO, CNPSA; IGOR R. SAVOLDI, UDESC/Chapecó; UNC/Concórdia; CEDISA; MONICA CORREA LEDUR, CNPSA. |
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Título: |
Stable reference genes for expression studies in breast muscle of normal and white striping-affected chickens. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Molecular Biology Reports, 2019. |
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DOI: |
10.1007/s11033-019-05103-z |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The normalization with proper reference genes is a crucial step to obtain accurate mRNA expression levels in quantitative PCR (qPCR) studies. Therefore, in this study, 10 reference candidate genes were evaluated to determine their stability in normal pectoralis major muscle of broilers and those counterparts afected with White Striping (WS) myopathy at 42 days age. Four diferent tools were used for ranking the most stable genes: GeNorm, NormFinder, BestKeeper and Comparative Ct (ΔCt), and a general ranking was performed using the RankAggreg tool to select the best reference genes among all tools. From the 10 genes evaluated in the breast muscle of broilers, 8 were amplifed. Most of the algorithms/tools indicated the same two genes, RPL30 and RPL5, as the most stable in the broilers breast muscle. In addition, there was agreement among the tools for the least stable genes: MRPS27, GAPDH and RPLP1 in the broilers breast muscle. Therefore, it is interesting to note that even with diferent tools for evaluating gene expression, there was consensus on the most and least stable genes. These results indicate that the Ribosomal protein L30 (RPL30) and Ribosomal protein L5 (RPL5) can be recommended for accurate normalization in qPCR studies with chicken pectoralis major muscle afected with White Striping and other myopathies |
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Thesagro: |
Frango de Corte; Melhoramento Genético Animal. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02063naa a2200229 a 4500
001 2115458
005 2019-11-28
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1007/s11033-019-05103-z$2DOI
100 1 $aMARCIANO, C. M. M.
245 $aStable reference genes for expression studies in breast muscle of normal and white striping-affected chickens.$h[electronic resource]
260 $c2019
520 $aAbstract: The normalization with proper reference genes is a crucial step to obtain accurate mRNA expression levels in quantitative PCR (qPCR) studies. Therefore, in this study, 10 reference candidate genes were evaluated to determine their stability in normal pectoralis major muscle of broilers and those counterparts afected with White Striping (WS) myopathy at 42 days age. Four diferent tools were used for ranking the most stable genes: GeNorm, NormFinder, BestKeeper and Comparative Ct (ΔCt), and a general ranking was performed using the RankAggreg tool to select the best reference genes among all tools. From the 10 genes evaluated in the breast muscle of broilers, 8 were amplifed. Most of the algorithms/tools indicated the same two genes, RPL30 and RPL5, as the most stable in the broilers breast muscle. In addition, there was agreement among the tools for the least stable genes: MRPS27, GAPDH and RPLP1 in the broilers breast muscle. Therefore, it is interesting to note that even with diferent tools for evaluating gene expression, there was consensus on the most and least stable genes. These results indicate that the Ribosomal protein L30 (RPL30) and Ribosomal protein L5 (RPL5) can be recommended for accurate normalization in qPCR studies with chicken pectoralis major muscle afected with White Striping and other myopathies
650 $aFrango de Corte
650 $aMelhoramento Genético Animal
700 1 $aIBELLI, A. M. G.
700 1 $aPEIXOTO, J. de O.
700 1 $aSAVOLDI, I. R.
700 1 $aCARMO, C. B. do
700 1 $aFERNANDES, L. T.
700 1 $aLEDUR, M. C.
773 $tMolecular Biology Reports, 2019.
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Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
25/10/2017 |
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Data da última atualização: |
08/02/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
FIGUEIREDO, R. de O.; PEREIRA, A. S.; COSTA, C. F. G.; TORRESAN, F. E.; LEIVAS, J. F.; FILIZOLA, H. F.; SONODA, K. C.; REIS, L. C.; SOUZA, M. D. de; GOMES, M. A. F.; ZUCCARI, M. L.; SILVA, M. S. G. M. e; PICCOLO, M. C.; TANK, M.; CRUZ, P. P. N.; PAMPLIN, P. A. Z.; CAMARGO, P. B. de; BOEIRA, R. C.; BARIZON, R. R. M.; PEREIRA, S. E. M.; PAZIANOTTO, R. A. A.; GREEN, T. R.; FERRACINI, V. L. |
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Afiliação: |
RICARDO DE OLIVEIRA FIGUEIREDO, CNPMA; ANDERSON SOARES PEREIRA, CNPMA; C. F. G. COSTA; FABIO ENRIQUE TORRESAN, CNPM; JANICE FREITAS LEIVAS, CNPM; HELOISA FERREIRA FILIZOLA, CNPMA; KATHIA CRISTHINA SONODA, CNPMA; L. C. REIS; MANOEL DORNELAS DE SOUZA, CNPMA; MARCO ANTONIO FERREIRA GOMES, CNPMA; MARIA LUCIA ZUCCARI, CNPMA; MARIANA SILVEIRA GUERRA MOURA E SIL, CNPMA; M. C. PICCOLO; M. TANK; P. P. N. CRUZ; P. A. Z. PAMPLIN; P. B. de CAMARGO; RITA CARLA BOEIRA, CNPMA; ROBSON ROLLAND MONTICELLI BARIZON, CNPMA; SANDRO EDUARDO MARSCHHAUSEN PEREIRA, CNPMA; RICARDO ANTONIO ALMEIDA PAZIANOTTO, CNPMA; T. R. GREEN; VERA LUCIA FERRACINI, CNPMA. |
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Título: |
Land use effects on the ecohydrology in headwater areas of the Jaguari river basin. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: INTERNACIONAL CONFERENCE ON ECOHYDROLOGY FOR SECURITY OF AQUATIC ECOSYSTEMS AND SOCIETIES, 2017, Campos dos Goytacazes. Book of abstracts... Campos dos Goytacazes: UENF, 2017. |
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Idioma: |
Português |
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Conteúdo: |
The headwaters of the Jaguari river basin are strategically important to the water resources in the largest reservoir system of the São Paulo state, called ?Cantareira?, which has recently suffered the largest water crisis in its history. Investigation at small watershed scales is a valuable tool to understand land-water interactions and their associated hvdrological processes. Our research approach included evaluations of streamwater uality and quantity, aquatic biota, overland flow, solls, land use, and land management As part of the region is under a conservation program of payment for environmental services (PES),we also examine the efficiency of this public policy. In addition, our study could help guide the agricultural management of rural properties. We detected that water quality drastically decreased downstream in the main channels of the basin and that the reforestation has improved some water quality parameters over time. Moreover, using macroinvertebrates as bioindicators we observed a trend of stream aquatic biota recovery at a catchment under the PES program comparing with a quasi-pristine stream and another stream under impacts from careless agriculture practices. Besides this, some preliminary results demonstrate aneed to regulate the use of pesticides at some agricultural areas; also considering the potential of sediment and water transfer from the steep slopes to the stream, improved management for farm soil and water conservation is needed urgently in this partof the basin. Such management together with PESprograms can help to maintain the sustainability of the water resources of the ?Cantareira? under increased rainfall variability. MenosThe headwaters of the Jaguari river basin are strategically important to the water resources in the largest reservoir system of the São Paulo state, called ?Cantareira?, which has recently suffered the largest water crisis in its history. Investigation at small watershed scales is a valuable tool to understand land-water interactions and their associated hvdrological processes. Our research approach included evaluations of streamwater uality and quantity, aquatic biota, overland flow, solls, land use, and land management As part of the region is under a conservation program of payment for environmental services (PES),we also examine the efficiency of this public policy. In addition, our study could help guide the agricultural management of rural properties. We detected that water quality drastically decreased downstream in the main channels of the basin and that the reforestation has improved some water quality parameters over time. Moreover, using macroinvertebrates as bioindicators we observed a trend of stream aquatic biota recovery at a catchment under the PES program comparing with a quasi-pristine stream and another stream under impacts from careless agriculture practices. Besides this, some preliminary results demonstrate aneed to regulate the use of pesticides at some agricultural areas; also considering the potential of sediment and water transfer from the steep slopes to the stream, improved management for farm soil and water conservation is needed urgently in this... Mostrar Tudo |
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Palavras-Chave: |
Agricultural watershed; Hydrobiogeochemistry; Water crisis. |
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Thesaurus NAL: |
ecosystem services; reforestation; water conservation. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/165511/1/RA-FigueiredoRO-et-al-IntlConferenceOnEcohidrology...-2017-LandUse...-PA....pdf
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Marc: |
LEADER 03079nam a2200445 a 4500
001 2078102
005 2018-02-08
008 2017 bl uuuu u00u1 u #d
100 1 $aFIGUEIREDO, R. de O.
245 $aLand use effects on the ecohydrology in headwater areas of the Jaguari river basin.$h[electronic resource]
260 $aIn: INTERNACIONAL CONFERENCE ON ECOHYDROLOGY FOR SECURITY OF AQUATIC ECOSYSTEMS AND SOCIETIES, 2017, Campos dos Goytacazes. Book of abstracts... Campos dos Goytacazes: UENF$c2017
520 $aThe headwaters of the Jaguari river basin are strategically important to the water resources in the largest reservoir system of the São Paulo state, called ?Cantareira?, which has recently suffered the largest water crisis in its history. Investigation at small watershed scales is a valuable tool to understand land-water interactions and their associated hvdrological processes. Our research approach included evaluations of streamwater uality and quantity, aquatic biota, overland flow, solls, land use, and land management As part of the region is under a conservation program of payment for environmental services (PES),we also examine the efficiency of this public policy. In addition, our study could help guide the agricultural management of rural properties. We detected that water quality drastically decreased downstream in the main channels of the basin and that the reforestation has improved some water quality parameters over time. Moreover, using macroinvertebrates as bioindicators we observed a trend of stream aquatic biota recovery at a catchment under the PES program comparing with a quasi-pristine stream and another stream under impacts from careless agriculture practices. Besides this, some preliminary results demonstrate aneed to regulate the use of pesticides at some agricultural areas; also considering the potential of sediment and water transfer from the steep slopes to the stream, improved management for farm soil and water conservation is needed urgently in this partof the basin. Such management together with PESprograms can help to maintain the sustainability of the water resources of the ?Cantareira? under increased rainfall variability.
650 $aecosystem services
650 $areforestation
650 $awater conservation
653 $aAgricultural watershed
653 $aHydrobiogeochemistry
653 $aWater crisis
700 1 $aPEREIRA, A. S.
700 1 $aCOSTA, C. F. G.
700 1 $aTORRESAN, F. E.
700 1 $aLEIVAS, J. F.
700 1 $aFILIZOLA, H. F.
700 1 $aSONODA, K. C.
700 1 $aREIS, L. C.
700 1 $aSOUZA, M. D. de
700 1 $aGOMES, M. A. F.
700 1 $aZUCCARI, M. L.
700 1 $aSILVA, M. S. G. M. e
700 1 $aPICCOLO, M. C.
700 1 $aTANK, M.
700 1 $aCRUZ, P. P. N.
700 1 $aPAMPLIN, P. A. Z.
700 1 $aCAMARGO, P. B. de
700 1 $aBOEIRA, R. C.
700 1 $aBARIZON, R. R. M.
700 1 $aPEREIRA, S. E. M.
700 1 $aPAZIANOTTO, R. A. A.
700 1 $aGREEN, T. R.
700 1 $aFERRACINI, V. L.
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